Protein Sequencing Protocols
Bryan John Smith
A timely collection of current and state-of-the-art techniques for protein and peptide preparation and sequencing-all described by hands-on, masters of the procedures. Conveys valuable lessons and insights into using automated sequencers and mass spectrometers, preparing proteins and peptides on a microgram and submicrogram scale, mapping peptides, analyzing amino acids by pre- and post-column derivatization methods, and sequencing N- and C-terminal peptides by classical and newly developed techniques. Also deals with such troublesome problems as identifying sites of modification and working with very small samples.
年:
1997
版本:
1
出版商:
Springer
語言:
english
頁數:
368
ISBN 10:
0896033538
ISBN 13:
9780896033535
系列:
Methods in Molecular Biology 064
文件:
PDF, 19.66 MB
IPFS:
,
english, 1997
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